Year: 2019 | Month: June | Volume 6 | Issue 1
In Vitro Multiplication from Nodal Explant of Roseapple and Genetic Purity Study of Conserved Plantlets Using Molecular Marker
Argha Chakraborty* and Prakash Kanti Das
DOI:10.30954/2347-9655.01.2019.2
Abstract:
In vitro multiplication, an exceptional replacement for conventional propagation practices as it produces maximum plantlets in minimum space and time. In vitro multiplication in rose apple was attempted through direct organogenesis of nodal explant. During shoot multiplication the highest numbers of shoots with the maximum number of leaves were achieved in full MS as well as in half MS with 4 mg/l BAP supplementation but half MS were recommended for better survival of plants as it lowered the polyphenol exudates interfering plant survival. In vitro root induction was achieved within two weeks of shootlet cultured on half MS with IBA at 6 mg/l concentration. Shoot multiplication from nodal explant produced on an average 30 plantlets from a single seed within 4-5 months. In vitro produced as well as conserved plantlets showed no genetic differences when it is compared to in vivo regenerated plants by using RAPD markers.
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