Year: 2016 | Month: June | Volume 3 | Issue 1
Molecular diversity of organophosphorus degrading bacteria from different field soils
R. Gireesh Kumar
A. Vijaya Gopal
R. Subhash Reddy
CH. V. Durga Rani
S. Triveni and K. Damodara Chari
DOI:10.5958/2454-9541.2016.00003.7
Abstract:
Sixteen bacterial isolates were obtained from insecticide treated tomato, brinjal maize and ground nut rhizospheric soils by enriching Mineral Salt Medium broth with supplement of chlorpyrifos, Phorate source. These isolates were characterized on the basis of cell morphology, cultural and biochemical properties. Among the eight chlorpyrifos degrading bacterial isolates, CDB-1isolate utilized the more pesticide. Among the eight phorate degrading bacterial isolates, the Phorate degrading bacteria (PDB)-1 isolate utilized the pesticide (Phorate) effectively. Degrading efficiency of isolates was determined by measuring the chlorpyrifos, phorate residual concentrations at intervals using Gas chromatographic method. Among the molecular diversity of 16 organophosphorus pesticide degrading isolates viz., chlorpyrifos degrading isolates 8, phorate degrading isolates 8 were also studied by PCR-RAPD technique. CDB7 shows separate lane with 26 per cent similarity compared to other isolates. CDB5 isolate shows 29 per cent similar when compared with other samples. CDB3 and CDB4 reveal same genetic relatedness according to dendrogram. CDB2 and CDB6 showed 54 percent similarity and CDB1 shows 41 percent similarity. CDB3 and CDB4 isolates are more or less similar in the genome and functional properties. Whereas, CDB7 isolate was highly diverse among other isolates.. PDB5 and PDB6 showed 55 per cent similar in coefficient scale. Whereas PDB2 and PDB3 revealed 75 per cent relatedness. The other isolates, PDB1, PDB7 and PDB4 shows 64-66 per cent similar. The PDB8 was highly diverse from remaining other seven isolates. PDB2 and PDB3 showed similar genome and functional properties.
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