Aims: The aim of this study is to compare the effect of bitter leaf (Vernonia Amygdalina) and glibenclamide in the treatment of diabetes in Alloxan-induced diabetic albino wistar rats. Materials and Methods: A total of 24 adult albino rats were used for the study. The rats were grouped into three groups of eight animals per group. Group A received 0.5 ml of distilled water and served as the control. Group B received 10 mg/kg/body weight of glibenclamide. Group C received 100 mg/kg/ body weight of bitter leaf extract. Effect of bitter leaf and glibenclamide were observed at every 2 days for up to 2 weeks. Results: The result showed that the extract caused a decrease in blood glucose level on the fourth day, but this was not statistically significant. Also, glibenclamide caused a decrease in the blood glucose level on the third day; this decrease was not statistically significant but slightly significant on the fourth day. On the sixth day, the extract caused a slightly significant decrease but when compared with the sixth day of glibenclamide, the decrease was very significant. From the eight-day to the fourteenth day, the decrease in the blood glucose levels for group B and C became very significant. However, group C (glibenclamide) showed highly significant decrease when compared with group B (extract). Glibenclamide showed significant (p<0.05 or p<0.01) decrease in mean blood glucose level from day 8 to till end (day 14) as compared to bitter leaf extract. Conclusion: The work suggests that Conclusion: The work suggests that glibenclamide has a more potent hypoglycemic effect in alloxan-induced diabetic wistar rats when compared with the extract and may be more effective in the treatment of diabetics.